Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
YSI™ Nitrate Calibration Solutions
Extremely accurate calibration solutions to calibrate field instruments equipped with a nitrate sensor
| Quantity | 1 Ea. |
|---|
GVS™ Dilution Bottle Phosphate Buffer Magnesium Chloride 99mL
Phosphate Buffer with magnesium chloride is used as the diluents for the preparation of dilutions in plate counts in the dairy and food industries. Recommended by ALHA for the recovery of injured Microorganisms from dairy and food samples. For use in BOD testing of water and wastewater.
| Formulation | Tris Glycine-SDS Buffer, 10X with 0.25M Tris base, 1.92M Glycine, 1% SDS |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Protein Sample Loading Buffer |
| Recommended Storage | 18° to 26°C |
| Concentration | 10X |
Lonza™ AccuGENE™ PBS
Ready to use solutions ideal for a wide range of molecular biology applications
| pH | 7.4 |
|---|---|
| Quantity | 1000mL |
| Recommended Storage | 18° to 26°C |
| For Use With (Application) | Ideal for a wide range of molecular biology applications |
GVS™ Dilution Bottle Butterfield's Buffer (72Pack)
Butterfield’s Buffer in 90 mL or 99 mL sterile bottles for 1:10 dilutions and general use.
| pH | 7.2 |
|---|---|
| Chemical Name or Material | Butterfield's Buffer |
Phosphate Buffered Saline 47, Gold Standard Diagnostics
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Ready-to-use buffer solution for AIX1000 workflows, supporting consistent assay performance and reliable diagnostic results.
Tris EDTA buffer, 1X, Molecular Biology Grade, Promega™
10mM Tris-HCl containing 1mM EDTA-Na2.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Recommended Storage | 15 to 30°C |
|---|
Tris-HCl, Molecular Grade, Promega™
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
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| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
HEPES (Free Acid), Promega
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| PubChem CID | 23831 |
|---|---|
| CAS | 7365-45-9 |
| Molecular Weight (g/mol) | 238.30 |
| ChEBI | CHEBI:42334 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
Molecular Grade 10X TAE Buffer, Promega
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Hoefer, Inc Tris Buffered Saline (TBS), 20X Solution (pH 7.4), Hoefer™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TBS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | TBS |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Sigma Aldrich Fine Chemicals Biosciences Trizmal™ Buffer, Sigma-Aldrich™
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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TAE Buffer, 10X Solution, Hoefer™, Ultrapure™
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Grade 10X TBE Buffer, Promega
Used for polyacrylamide and agarose gel electrophoresis.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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