Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
AccuGENE™ TAE Buffers, Lonza™
Formulated to match Lonza's Reliant™ and Latitude™ gels
AccuGENE™ 10X Tris-Glycine-SDS Buffers, Lonza™
For use with denaturing protein electrophoresis
GVS™ Dilution Bottle Phosphate Buffer Magnesium Chloride 99mL
Phosphate Buffer with magnesium chloride is used as the diluents for the preparation of dilutions in plate counts in the dairy and food industries. Recommended by ALHA for the recovery of injured Microorganisms from dairy and food samples. For use in BOD testing of water and wastewater.
YSI™ Nitrate Calibration Solutions
Extremely accurate calibration solutions to calibrate field instruments equipped with a nitrate sensor
AccuGENE™ 1X TE Buffer, Lonza™
A ready to use solution ideal for a wide range of molecular biology applications
Lonza™ AccuGENE™ PBS
Ready to use solutions ideal for a wide range of molecular biology applications
AccuGENE™ 10X Tris-Glycine Buffers, Lonza™
For use with denaturing protein electrophoresis
GVS™ Dilution Bottle Butterfield's Buffer (72Pack)
Butterfield’s Buffer in 90mL or 99mL prefilled sterile dilution bottles for easy 1:10 dilutions. Used extensively in the food, dairy, and pharmaceutical industries as a general diluent. Contains monobasic potassium phosphate.
HEPES (Free Acid), Promega
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
Hoefer, Inc Tris Buffered Saline (TBS), 20X Solution (pH 7.4), Hoefer™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TBS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
HEPES, Molecular Biology Grade (free acid), Promega
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
