Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Tris(hydroxymethyl)aminomethane, ACS Reagent (Tris; THAM; 2-Amino-2-(hydroxymethyl)-1,3-propanediol), Reagents
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tromethamine IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: NC(CO)(CO)CO
AccuGENE™ TAE Buffers, Lonza™
Formulated to match Lonza's Reliant™ and Latitude™ gels
AccuGENE™ 10X Tris-Glycine Buffers, Lonza™
For use with denaturing protein electrophoresis
GVS™ Dilution Bottle Butterfield's Buffer (72Pack)
Butterfield’s Buffer in 90mL or 99mL prefilled sterile dilution bottles for easy 1:10 dilutions. Used extensively in the food, dairy, and pharmaceutical industries as a general diluent. Contains monobasic potassium phosphate.
AccuGENE™ 10X Tris-Glycine-SDS Buffers, Lonza™
For use with denaturing protein electrophoresis
Lonza™ AccuGENE™ PBS
Ready to use solutions ideal for a wide range of molecular biology applications
YSI™ Nitrate Calibration Solutions
Extremely accurate calibration solutions to calibrate field instruments equipped with a nitrate sensor
GVS™ Dilution Bottle Phosphate Buffer Magnesium Chloride 99mL
Phosphate Buffer with magnesium chloride is used as the diluents for the preparation of dilutions in plate counts in the dairy and food industries. Recommended by ALHA for the recovery of injured Microorganisms from dairy and food samples. For use in BOD testing of water and wastewater.
AccuGENE™ 1X TE Buffer, Lonza™
A ready to use solution ideal for a wide range of molecular biology applications
Thermo Scientific™ TBS Buffer
Tris Buffered Saline (TBS) Buffer is supplied as a lyophilized powder and is intended for use with PanPath products.
Thermo Scientific™ Wash Buffer for GeneJET Gel Extraction Kit
Thermo Scientific GeneJET Wash Buffer is a component of the GeneJET Gel Extraction Kit and may be purchased separately. Provided in 45 mL aliquots.
